Mechanisms and enzymes involved in SARS coronavirus genome expression.
Identifieur interne : 005F35 ( Main/Exploration ); précédent : 005F34; suivant : 005F36Mechanisms and enzymes involved in SARS coronavirus genome expression.
Auteurs : Volker Thiel [Allemagne] ; Konstantin A. Ivanov [Allemagne] ; Ákos Putics [Allemagne] ; Tobias Hertzig [Allemagne] ; Barbara Schelle [Allemagne] ; Sonja Bayer [Allemagne] ; Benedikt Wei Brich [Allemagne] ; Eric J. Snijder [Pays-Bas] ; Holger Rabenau [Allemagne] ; Hans Wilhelm Doerr [Allemagne] ; Alexander E. Gorbalenya [Pays-Bas] ; John Ziebuhr [Allemagne]Source :
- The Journal of general virology [ 0022-1317 ] ; 2003.
Descripteurs français
- KwdFr :
- ARN messager (), ARN messager (génétique), Alignement de séquences, Biosynthèse des protéines, Conformation d'acide nucléique, Cysteine endopeptidases (génétique), Cysteine endopeptidases (métabolisme), Domaine catalytique, Données de séquences moléculaires, Décalage ribosomique du cadre de lecture, Génome viral, Papaïne (génétique), Papaïne (métabolisme), Protéines virales (génétique), Protéines virales (métabolisme), RNA helicases (biosynthèse), RNA helicases (génétique), Régulation de l'expression des gènes viraux, Séquence d'acides aminés, Virus du SRAS (enzymologie), Virus du SRAS (génétique), Virus du SRAS (isolement et purification).
- MESH :
- biosynthèse : RNA helicases.
- enzymologie : Virus du SRAS.
- génétique : ARN messager, Cysteine endopeptidases, Papaïne, Protéines virales, RNA helicases, Virus du SRAS.
- isolement et purification : Virus du SRAS.
- métabolisme : Cysteine endopeptidases, Papaïne, Protéines virales.
- ARN messager, Alignement de séquences, Biosynthèse des protéines, Conformation d'acide nucléique, Domaine catalytique, Données de séquences moléculaires, Décalage ribosomique du cadre de lecture, Génome viral, Régulation de l'expression des gènes viraux, Séquence d'acides aminés.
English descriptors
- KwdEn :
- Amino Acid Sequence, Catalytic Domain, Cysteine Endopeptidases (genetics), Cysteine Endopeptidases (metabolism), Frameshifting, Ribosomal, Gene Expression Regulation, Viral, Genome, Viral, Molecular Sequence Data, Nucleic Acid Conformation, Papain (genetics), Papain (metabolism), Protein Biosynthesis, RNA Helicases (biosynthesis), RNA Helicases (genetics), RNA, Messenger (chemistry), RNA, Messenger (genetics), SARS Virus (enzymology), SARS Virus (genetics), SARS Virus (isolation & purification), Sequence Alignment, Viral Proteins (genetics), Viral Proteins (metabolism).
- MESH :
- chemical , biosynthesis : RNA Helicases.
- chemical , chemistry : RNA, Messenger.
- chemical , genetics : Cysteine Endopeptidases, Papain, RNA Helicases, RNA, Messenger, Viral Proteins.
- chemical , metabolism : Cysteine Endopeptidases, Papain, Viral Proteins.
- enzymology : SARS Virus.
- genetics : SARS Virus.
- isolation & purification : SARS Virus.
- Amino Acid Sequence, Catalytic Domain, Frameshifting, Ribosomal, Gene Expression Regulation, Viral, Genome, Viral, Molecular Sequence Data, Nucleic Acid Conformation, Protein Biosynthesis, Sequence Alignment.
Abstract
A novel coronavirus is the causative agent of the current epidemic of severe acute respiratory syndrome (SARS). Coronaviruses are exceptionally large RNA viruses and employ complex regulatory mechanisms to express their genomes. Here, we determined the sequence of SARS coronavirus (SARS-CoV), isolate Frankfurt 1, and characterized key RNA elements and protein functions involved in viral genome expression. Important regulatory mechanisms, such as the (discontinuous) synthesis of eight subgenomic mRNAs, ribosomal frameshifting and post-translational proteolytic processing, were addressed. Activities of three SARS coronavirus enzymes, the helicase and two cysteine proteinases, which are known to be critically involved in replication, transcription and/or post-translational polyprotein processing, were characterized. The availability of recombinant forms of key replicative enzymes of SARS coronavirus should pave the way for high-throughput screening approaches to identify candidate inhibitors in compound libraries.
DOI: 10.1099/vir.0.19424-0
PubMed: 12917450
Affiliations:
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Ivanov</name><affiliation wicri:level="3"><nlm:affiliation>Institute of Virology and Immunology, University of Würzburg, Versbacher Str. 7, 97078 Würzburg, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Virology and Immunology, University of Würzburg, Versbacher Str. 7, 97078 Würzburg</wicri:regionArea><placeName><region type="land" nuts="1">Bavière</region><region type="district" nuts="2">District de Basse-Franconie</region><settlement type="city">Wurtzbourg</settlement></placeName></affiliation></author><author><name sortKey="Putics, Akos" sort="Putics, Akos" uniqKey="Putics A" first="Ákos" last="Putics">Ákos Putics</name><affiliation wicri:level="3"><nlm:affiliation>Institute of Virology and Immunology, University of Würzburg, Versbacher Str. 7, 97078 Würzburg, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Virology and Immunology, University of Würzburg, Versbacher Str. 7, 97078 Würzburg</wicri:regionArea><placeName><region type="land" nuts="1">Bavière</region><region type="district" nuts="2">District de Basse-Franconie</region><settlement type="city">Wurtzbourg</settlement></placeName></affiliation></author><author><name sortKey="Hertzig, Tobias" sort="Hertzig, Tobias" uniqKey="Hertzig T" first="Tobias" last="Hertzig">Tobias Hertzig</name><affiliation wicri:level="3"><nlm:affiliation>Institute of Virology and Immunology, University of Würzburg, Versbacher Str. 7, 97078 Würzburg, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Virology and Immunology, University of Würzburg, Versbacher Str. 7, 97078 Würzburg</wicri:regionArea><placeName><region type="land" nuts="1">Bavière</region><region type="district" nuts="2">District de Basse-Franconie</region><settlement type="city">Wurtzbourg</settlement></placeName></affiliation></author><author><name sortKey="Schelle, Barbara" sort="Schelle, Barbara" uniqKey="Schelle B" first="Barbara" last="Schelle">Barbara Schelle</name><affiliation wicri:level="3"><nlm:affiliation>Institute of Virology and Immunology, University of Würzburg, Versbacher Str. 7, 97078 Würzburg, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Virology and Immunology, University of Würzburg, Versbacher Str. 7, 97078 Würzburg</wicri:regionArea><placeName><region type="land" nuts="1">Bavière</region><region type="district" nuts="2">District de Basse-Franconie</region><settlement type="city">Wurtzbourg</settlement></placeName></affiliation></author><author><name sortKey="Bayer, Sonja" sort="Bayer, Sonja" uniqKey="Bayer S" first="Sonja" last="Bayer">Sonja Bayer</name><affiliation wicri:level="3"><nlm:affiliation>Institute of Virology and Immunology, University of Würzburg, Versbacher Str. 7, 97078 Würzburg, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Virology and Immunology, University of Würzburg, Versbacher Str. 7, 97078 Würzburg</wicri:regionArea><placeName><region type="land" nuts="1">Bavière</region><region type="district" nuts="2">District de Basse-Franconie</region><settlement type="city">Wurtzbourg</settlement></placeName></affiliation></author><author><name sortKey="Wei Brich, Benedikt" sort="Wei Brich, Benedikt" uniqKey="Wei Brich B" first="Benedikt" last="Wei Brich">Benedikt Wei Brich</name><affiliation wicri:level="3"><nlm:affiliation>Institute of Virology and Immunology, University of Würzburg, Versbacher Str. 7, 97078 Würzburg, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Virology and Immunology, University of Würzburg, Versbacher Str. 7, 97078 Würzburg</wicri:regionArea><placeName><region type="land" nuts="1">Bavière</region><region type="district" nuts="2">District de Basse-Franconie</region><settlement type="city">Wurtzbourg</settlement></placeName></affiliation></author><author><name sortKey="Snijder, Eric J" sort="Snijder, Eric J" uniqKey="Snijder E" first="Eric J" last="Snijder">Eric J. Snijder</name><affiliation wicri:level="3"><nlm:affiliation>Molecular Virology Laboratory, Department of Medical Microbiology, Leiden University Medical Center, Leiden, The Netherlands.</nlm:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Molecular Virology Laboratory, Department of Medical Microbiology, Leiden University Medical Center, Leiden</wicri:regionArea><placeName><settlement type="city">Leyde</settlement><region nuts="2" type="province">Hollande-Méridionale</region></placeName></affiliation></author><author><name sortKey="Rabenau, Holger" sort="Rabenau, Holger" uniqKey="Rabenau H" first="Holger" last="Rabenau">Holger Rabenau</name><affiliation wicri:level="1"><nlm:affiliation>Institute for Medical Virology, Johann Wolfgang Goethe University, Frankfurt (Main), Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute for Medical Virology, Johann Wolfgang Goethe University, Frankfurt (Main)</wicri:regionArea><wicri:noRegion>Frankfurt (Main)</wicri:noRegion><wicri:noRegion>Frankfurt (Main)</wicri:noRegion><wicri:noRegion>Frankfurt (Main)</wicri:noRegion></affiliation></author><author><name sortKey="Doerr, Hans Wilhelm" sort="Doerr, Hans Wilhelm" uniqKey="Doerr H" first="Hans Wilhelm" last="Doerr">Hans Wilhelm Doerr</name><affiliation wicri:level="1"><nlm:affiliation>Institute for Medical Virology, Johann Wolfgang Goethe University, Frankfurt (Main), Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute for Medical Virology, Johann Wolfgang Goethe University, Frankfurt (Main)</wicri:regionArea><wicri:noRegion>Frankfurt (Main)</wicri:noRegion><wicri:noRegion>Frankfurt (Main)</wicri:noRegion><wicri:noRegion>Frankfurt (Main)</wicri:noRegion></affiliation></author><author><name sortKey="Gorbalenya, Alexander E" sort="Gorbalenya, Alexander E" uniqKey="Gorbalenya A" first="Alexander E" last="Gorbalenya">Alexander E. Gorbalenya</name><affiliation wicri:level="3"><nlm:affiliation>Molecular Virology Laboratory, Department of Medical Microbiology, Leiden University Medical Center, Leiden, The Netherlands.</nlm:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Molecular Virology Laboratory, Department of Medical Microbiology, Leiden University Medical Center, Leiden</wicri:regionArea><placeName><settlement type="city">Leyde</settlement><region nuts="2" type="province">Hollande-Méridionale</region></placeName></affiliation></author><author><name sortKey="Ziebuhr, John" sort="Ziebuhr, John" uniqKey="Ziebuhr J" first="John" last="Ziebuhr">John Ziebuhr</name><affiliation wicri:level="3"><nlm:affiliation>Institute of Virology and Immunology, University of Würzburg, Versbacher Str. 7, 97078 Würzburg, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Virology and Immunology, University of Würzburg, Versbacher Str. 7, 97078 Würzburg</wicri:regionArea><placeName><region type="land" nuts="1">Bavière</region><region type="district" nuts="2">District de Basse-Franconie</region><settlement type="city">Wurtzbourg</settlement></placeName></affiliation></author></analytic><series><title level="j">The Journal of general virology</title><idno type="ISSN">0022-1317</idno><imprint><date when="2003" type="published">2003</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Catalytic Domain</term><term>Cysteine Endopeptidases (genetics)</term><term>Cysteine Endopeptidases (metabolism)</term><term>Frameshifting, Ribosomal</term><term>Gene Expression Regulation, Viral</term><term>Genome, Viral</term><term>Molecular Sequence Data</term><term>Nucleic Acid Conformation</term><term>Papain (genetics)</term><term>Papain (metabolism)</term><term>Protein Biosynthesis</term><term>RNA Helicases (biosynthesis)</term><term>RNA Helicases (genetics)</term><term>RNA, Messenger (chemistry)</term><term>RNA, Messenger (genetics)</term><term>SARS Virus (enzymology)</term><term>SARS Virus (genetics)</term><term>SARS Virus (isolation & purification)</term><term>Sequence Alignment</term><term>Viral Proteins (genetics)</term><term>Viral Proteins (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN messager ()</term><term>ARN messager (génétique)</term><term>Alignement de séquences</term><term>Biosynthèse des protéines</term><term>Conformation d'acide nucléique</term><term>Cysteine endopeptidases (génétique)</term><term>Cysteine endopeptidases (métabolisme)</term><term>Domaine catalytique</term><term>Données de séquences moléculaires</term><term>Décalage ribosomique du cadre de lecture</term><term>Génome viral</term><term>Papaïne (génétique)</term><term>Papaïne (métabolisme)</term><term>Protéines virales (génétique)</term><term>Protéines virales (métabolisme)</term><term>RNA helicases (biosynthèse)</term><term>RNA helicases (génétique)</term><term>Régulation de l'expression des gènes viraux</term><term>Séquence d'acides aminés</term><term>Virus du SRAS (enzymologie)</term><term>Virus du SRAS (génétique)</term><term>Virus du SRAS (isolement et purification)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>RNA Helicases</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>RNA, Messenger</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Cysteine Endopeptidases</term><term>Papain</term><term>RNA Helicases</term><term>RNA, Messenger</term><term>Viral Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Cysteine Endopeptidases</term><term>Papain</term><term>Viral Proteins</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>RNA helicases</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN messager</term><term>Cysteine endopeptidases</term><term>Papaïne</term><term>Protéines virales</term><term>RNA helicases</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Cysteine endopeptidases</term><term>Papaïne</term><term>Protéines virales</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Catalytic Domain</term><term>Frameshifting, Ribosomal</term><term>Gene Expression Regulation, Viral</term><term>Genome, Viral</term><term>Molecular Sequence Data</term><term>Nucleic Acid Conformation</term><term>Protein Biosynthesis</term><term>Sequence Alignment</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>ARN messager</term><term>Alignement de séquences</term><term>Biosynthèse des protéines</term><term>Conformation d'acide nucléique</term><term>Domaine catalytique</term><term>Données de séquences moléculaires</term><term>Décalage ribosomique du cadre de lecture</term><term>Génome viral</term><term>Régulation de l'expression des gènes viraux</term><term>Séquence d'acides aminés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A novel coronavirus is the causative agent of the current epidemic of severe acute respiratory syndrome (SARS). Coronaviruses are exceptionally large RNA viruses and employ complex regulatory mechanisms to express their genomes. Here, we determined the sequence of SARS coronavirus (SARS-CoV), isolate Frankfurt 1, and characterized key RNA elements and protein functions involved in viral genome expression. Important regulatory mechanisms, such as the (discontinuous) synthesis of eight subgenomic mRNAs, ribosomal frameshifting and post-translational proteolytic processing, were addressed. Activities of three SARS coronavirus enzymes, the helicase and two cysteine proteinases, which are known to be critically involved in replication, transcription and/or post-translational polyprotein processing, were characterized. The availability of recombinant forms of key replicative enzymes of SARS coronavirus should pave the way for high-throughput screening approaches to identify candidate inhibitors in compound libraries.</div></front></TEI><affiliations><list><country><li>Allemagne</li><li>Pays-Bas</li></country><region><li>Bavière</li><li>District de Basse-Franconie</li><li>Hollande-Méridionale</li></region><settlement><li>Leyde</li><li>Wurtzbourg</li></settlement></list><tree><country name="Allemagne"><region name="Bavière"><name sortKey="Thiel, Volker" sort="Thiel, Volker" uniqKey="Thiel V" first="Volker" last="Thiel">Volker Thiel</name></region><name sortKey="Bayer, Sonja" sort="Bayer, Sonja" uniqKey="Bayer S" first="Sonja" last="Bayer">Sonja Bayer</name><name sortKey="Doerr, Hans Wilhelm" sort="Doerr, Hans Wilhelm" uniqKey="Doerr H" first="Hans Wilhelm" last="Doerr">Hans Wilhelm Doerr</name><name sortKey="Hertzig, Tobias" sort="Hertzig, Tobias" uniqKey="Hertzig T" first="Tobias" last="Hertzig">Tobias Hertzig</name><name sortKey="Ivanov, Konstantin A" sort="Ivanov, Konstantin A" uniqKey="Ivanov K" first="Konstantin A" last="Ivanov">Konstantin A. Ivanov</name><name sortKey="Putics, Akos" sort="Putics, Akos" uniqKey="Putics A" first="Ákos" last="Putics">Ákos Putics</name><name sortKey="Rabenau, Holger" sort="Rabenau, Holger" uniqKey="Rabenau H" first="Holger" last="Rabenau">Holger Rabenau</name><name sortKey="Schelle, Barbara" sort="Schelle, Barbara" uniqKey="Schelle B" first="Barbara" last="Schelle">Barbara Schelle</name><name sortKey="Wei Brich, Benedikt" sort="Wei Brich, Benedikt" uniqKey="Wei Brich B" first="Benedikt" last="Wei Brich">Benedikt Wei Brich</name><name sortKey="Ziebuhr, John" sort="Ziebuhr, John" uniqKey="Ziebuhr J" first="John" last="Ziebuhr">John Ziebuhr</name></country><country name="Pays-Bas"><region name="Hollande-Méridionale"><name sortKey="Snijder, Eric J" sort="Snijder, Eric J" uniqKey="Snijder E" first="Eric J" last="Snijder">Eric J. Snijder</name></region><name sortKey="Gorbalenya, Alexander E" sort="Gorbalenya, Alexander E" uniqKey="Gorbalenya A" first="Alexander E" last="Gorbalenya">Alexander E. Gorbalenya</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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